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KMID : 0900919990230030271
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Korean journal of Animal Reproduction
1999 Volume.23 No. 3 p.271 ~ p.278
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Cytogenetic Properties of Bovine Reconstituted Embryos by Cell Cycle-Controlled Nuclear Transfer
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Cheong H.-T.
Yang B.-K.
Park C.-K.
Kim C.-I.
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Abstract
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This study was conducted to investigate the cytogenetic properties, in vitro development, and their relationship in the bovine reconstituted embryos following cell cycle-controlled nuclear transfer. Sixteen-cell stage embryos were treated by nocodazole, and after release from nocodazole treatment, their blastomeres were separated and allowed to subsequent cleavage. Blastomeres within 1.5 h post cleavage(hpc) and at 3hpc were transferred to enucleated oocytes at MII-phase or S-phase. Donor nuclei transferred into M II-phase recipients underwent various nuclear remodeling, such as extrusion of a polar body(PB)-like structure, premature chromosome condensation(PCC) and chromatin modifications. These nuclear remodeling patterns varied by the time post cleavage of donor blastomeres. Developmental rate to the blastocyst stage differed with time post cleavage of donor blastomeres and existence of a PB-like structure. Whereas do-nor nuclei transferred into S-phase oocytes did not undergo PCC and other major modifications, and their developmental potentials less depended on the nuclei types. This result confirms that the nuclear remodeling type differs with donor and recipient cell cycle stage, which affect the development of reconstituted bovine embryos.
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KEYWORD
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Nuclear transfer, Cell cycle stage, Nuclear remodeling, In vitro development, Bovine embryo
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